The tour itinerary will unite the most significant places: the "ancient" church, the "new" church, both dedicated to Santa Maria delle Grazie, the Monastery and the new sanctuary. The ancient church of the mid-1500s has a very simple façade and inside it is composed of a single baroque style nave. At a second entrance to the convent is the cell of Padre Pio and the crypt where the body of Padre Pio was housed before being transferred to the nearby sanctuary. The visit continues with the "new" church, built for the explicit desire of Padre Pio, with the intention of being able to accommodate in an appropriate manner the considerable flow of thousands of pilgrims that flowed ever more numerous to San Giovanni Rotondo. The tour ends at the Sanctuary, designed by architect Renzo Piano, one of the largest churches in Italy, capable of holding up to 7,000 people.

San Giovanni Rotondo is the core of the spiritual soul of the Gargano, an important destination for devotional practices and religious pilgrimages.